Cell-free translation of maternal messenger RNA from sea urchin eggs.
نویسندگان
چکیده
The template activity of RNA extracted from unfertilized sea urchin eggs was demonstrated in a cell-free translation system, and, for the first time, specific proteins were identified among the products. All messenger RNA activity is recovered, under the conditions used, from the postribosomal supernatant. Histones, among many other proteins, were identified specifically as products of this "maternal" messenger RNA.
منابع مشابه
Inhibitor of eukaryotic initiation factor 4F activity in unfertilized sea urchin eggs.
Extracts from unfertilized sea urchin eggs contain an inhibitor of translation that inhibits protein synthesis in cell-free translation systems from sea urchin embryos or rabbit reticulocytes. The inhibitory effects of egg extracts can be reversed by the addition of mammalian eukaryotic initiation factor 4F (eIF-4F) in both sea urchin embryo and reticulocyte systems, suggesting that the inhibit...
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Ultrastructural studies on sea urchin eggs (Afzelius, 1957 ; Harris, 1967, 1969; Verhey and Moyer, 1967 ; Bal et al., 1968) have revealed certain cytoplasmic bodies, called heavy bodies, which originate from the egg nucleus. These structures are aggregations of approximately 150 A electron-opaque granules surrounded by annulate lamellae . It has been suggested that heavy bodies are cytoplasmic ...
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 70 9 شماره
صفحات -
تاریخ انتشار 1973